41.5 - 2.45 = 39.05 alkaline salts.
41.5 : 11.8 (c): : 2.45 : 0.6956 insoluble salts in 1000 urine; by (/) we found the earthy phosphates 0.65.
11.8 - 0.7 = 11.1 alkaline salts per 1000 urine.
(o) The insoluble portion dissolved in a little nitric acid, supersaturated with ammonia and redissolved in acetic acid, gave by oxalate of ammonia a precipitate which yielded on igni tion 1.32 grs. carbonate of lime.
2.45 : 0.6956 : : 1.32 : x (= 0.3753) carb. lime.
100 : 0.3753 : : 56 : x (= 0.2101) lime.
(p) The solution filtered from the oxalate of lime and supersaturated by ammonia gave, on agitation followed by repose for some hours, a crystalline precipitate, weighing 1.18 grs. after ignition.
2.45 : 0.6956 : : 1.18 : x (= 0.3345) 100 : 0.3345 : 35.71 : (=0.1198) mag nesia.
(q) 0.6956 - (0.2101 + 0.1198) = 0.3659. phosphoric acid with the earths.
0.3659 + 1.753 (m) 2.1189 total phos phoric acid in 1000.
(r) 15 grs. of the alkaline salts (n) were dis solved in water and converted into chlorides by admixture with chloride of barium in ex cess ; a precipitate of 6.4 grs. of sulphate and phosphate of baryta formed ; on treating this precipitate with nitric acid, 0.45 sulphate of baryta remained.
15 : 11.1 (n) : 0.45 : 0.333.
100 : 0.333 : : 34.19 : 0.114 sulphuric acid in the ash, from 1000 parts of urine.
But 1000 grs. of urine we found (by k) to contain 1.702 grs. sulphuric acid, therefore 1.702 - 0.114 = 1.588 grs. of sulphuric acid have been expelled by ignition.
(s) The filtered solution was heated with caustic and carbonated ammonia to precipitate the excess of baryta as carbonate. The whole filtered, evaporated to dryness, and ignited to expel the ammoniacal salts. The fixed chlo rides weighed 14 grs. They were dissolved in water, treated with bichloride of platinum, eva porated nearly to dryness by a water-bath, then treated with alcohol, the platino-chloride of potassium amounted to 13.40 grs.
15 : 11.1 : : 13.40 : 9.916.
100 : 9.916 : 19.43 : : x (= 1.926) potash.
(t) But 247, (1 eqt.platino-chlor.potassium) : 13.40 : : 76, (1 eqt.chlor. potassium)
: x (= 4.123) chloride potassium, and 14 - 4.123 = 9.877 chloride sodium.
15 : 11.1 : : 9.877 : x (= 7.3089) chloride sodium, and 60, (1 eqt.chlor. sodium) : 7.3089 : : 24 (1 eqt. sodium) ; x (= 2.9235) sodium.
(u) 10 grs. of the alkaline salts were dis solved in water, the solution acidulated with nitric acid, and nitrate of silver added in slight excess : the precipitate of chloride of silver amounted to 15.61 grs.
10 : 11.1 : : 15.61 : x (= 17.3271) 100 : 17.3271 : : 25 : (= 4.3317) chlo rine in the ashes of 1000 parts of urine. We find the equivalent quantity of sodium as follows : 36 : 4.3317 : : 24 : x (= 2.8878) sodium, equivalent to the chlorine.
The chloride of sodium therefore amounts to 7.2195 grs.; deducting the sodium com bined with chlorine from the entire quan tity in the urine (t), we obtain 2.92356 - 2.8878 = 0.03576, or 0.0536 soda.
(v) Now before ignition the chlorine (by i) amounted to 4.942 grs.
deduct.... 4.3317 combined with so - dium in the ash.
0.6103 chlorinevolatilized, probably in tbe form of muriate of am monia, the amount of which appears by the following calculation : 36 : 0.6103 : : 54 : s (= 0.9154) muriate ammonia.
The results of the analysis are here sub joined.
Water 956.8000 /Urea 14.2300 'Organic Uric acid 0.3700 matters & 129.8224 Alcoholic ext. 12.5270 ammoni- Watery ext 1.6050 acal salts 1 Vesical mucus 0.1650 Mur. ammonia 0.9154 Chlor. sodium 7.2195 Phosphc. acid 2.1189 Fixed sa- ) Sul ph uric acid 1.7020 line mat- ;13.1494 Lime 0.2101 ters Magnesia ... 0.1198 Potash 1.9260 Soda 0.0536 999.9623 In analysing diabetic urine the method must he modified, as will be seen by the following example.
It was feebly acid, and had a specific gravity of 1038.
Evaporated in vacuo over sulphuric acid, it furnished a pale amber-coloured soft mass, which weighed, On the third day .... 55.9 grs.
sixth . 54.1 ninth 53.4 fourteenth 53.1 thirtieth 52.2 The temperature varied between 60° and 7 and the vacuum shoved from 1 inch to 1 inches on the pressure gauge.