500 : 1000 : : 0•69 : x (= 1.38) salts bible in alcohol.
2.15 - (0.04 0.69) = 1.42.
500 : 1000 : : 1.42 : x (=.• 2.84) watery ex tract.
By calculation, as in the previous analysis, the alcoholic: extract is 15.98 grs.
The composition of the urine is thus deter mined to be Water 895.60 Fixed salts 3.09 Organic s Sugar U rea 80.29 2.12 matters & vola- Alcoholic extr. 15.98 tile salts Watery extract 2.84 Uric acid &muc. 0.08 1000.00 Where albumen occurs in the urine, we por ceed as in the following instance.
The fluid was rather turbid, feebly alkaline, and of sp. gr. 1013.1. It was found to contain 30.2 per 1000 of solid matter, of which 9.17 were salts and 21.03 organic vo latile matters.
500 grs. evaporated to dryness, and the residue finely powdered, taking care that none of the particles were lost (by placing the mortar on a large sheet of paper, and co vering the mouth, of it likewise with paper). It was treated with boiling water and washed as long as any thing dissolved. The insoluble portion collected On a filter, dried and weighed, amounted to 3.1 grs.
500 : 1000 : : 3.1 : x (= 6.2) albumen, with traces of uric acid.
The filtered liquid was evaporated to dry ness, and treated with alcohol and nitric acid for urea in the usual manner : the urea per 1000 = 4.72 grs.
The other ingredients were determined as usual and furnished the following results.
Water 969.80 Saline matters 9.17 Albumen and 6.20 °rganic 21. U uric acid .. 5 03 rea 4.72 matters 5 Alcoholic extract 8.43 Watery extract. . 1.68 1000.00 2. Analysis of the blood.
Unless present when the blood is drawn, we are obliged to proceed as in the following example, furnished from a patient suffering from a chronic cerebral affection.
(a) The entire blood employed amounted to 6735 grs.; it had been dmwn 24 hours, and had formed a tolerably firm but fiat coagulum, which weighed 3560 grs. The serum that had separated weighed 3175 grs., and had a sp. gr. of 1029.3.
The proportions of clot and scrum were therefore as follows, in 1000 parts.
6735 : 1000 : : 3560 : x (=528.6)coagulum.
6735 : 1000 : : 3175 : s (= 471.4) serum. The analysis divides itself into two portions, that of the scrum, and that of the clot.
Analysis of the serum.
(b) 200 grs. of scrum were dried at a tem perature of 212°. The residue amounted to 18.69grs.
200 : 1000 : : 18.69 : x (= 93.45) so/ids in serum.
'Therefore 1000 - 93.45 = 906.55, water in the serum.
(c) The residue was incinerated over the cir cular wicked spirit lamp, and amounted to 1.46 grs.
200 : 1000 : : 1.46 : x (= 7.3) saline matters.
(d) 500 grains of serum were dried in a pla tinum capsule, the dry mass carefully detached, pulverized, and digested in boiling ether, which was decanted and renewed three or four times. The ethereal solutions evaporated left 0.35 grs. of fatty matter ; of this cold alcohol dissolved 0.12 oily fat, leaving 0.23 of crystalline fats.
1000 parts of serum therefore contain 0.24 oily fht, and 0.46 crystallinefats.
(e) The undissolved residue was heated to expel adhering ether and treated with boiling water. It VMS thrown on a filter and washed repeatedly, until nitrate of silver produced an insignificant cloud when applied to a few drops of the washings. The filter and its con tents were dried ; when weighed the albumen amounted to 41.73 grs.
10 grs. when incinerated yielded 0.19 grs. of ash ; 10 : 41.73 : : 0.19 : (= 0.79287.) 41.73 - 0.793 = 40.937 ; 40.937 X 2 = 81.974 albumen.
(f) The filtered liquid evaporated weighed 4.66 grs. It was digested with alcohol, which was renewed as long as any thing was dis solved. The alcoholic solution evapomted amounted to 3.32 grs. (If urea, sugar, or bile be present, they will be contained in this extract and must be sought for in the usual manner.) 4.66 - 3.32 = 1.34 grs. watery extract, 1.34 x 2 = 2.68. I gr. of watery extract incinerated left 0.82 grs. of salts, and 1. : 2.68 : 0.82 : = 2.197, and 2.68 - 2.197 = 0.483 watery extract per 1000.
(g) 1.4 grs. of alcoholic extract, incinerated, left 1.00 grs. of saline matter.