Now 1.4 : 1 : : 6.64 : (= 4.74) saline matters, And 6.64 - 4.74 = 1.90 akoholic extract per 1000.
The serum therefore consists of Water 906.550 Fixed salts 7.300 Albumen 81.974 Alcoholic extract 1.900 Watery extract 0.483 Fats, oily 0.240 „ crystalline 0.460 998.907 _Analysis of the clot.
(h) 1000 grs. shred finely with a sharp knife were tied in a piece of calico and washed till colourless under a gentle stream of water. The fibrin tbat remained carefully dried at 212° weighed 5.67 grs.
Ether dissolved from this 0.07 grs. of oily fat. The pure fibrin therefore amou n ted to 5.60 grs. 1000 : 528.6 : : 5.6 : .r (= 2.962)fibrin. 1000: 528.6 by (a) : : 0.07 : x (= 0.04) fat from fibrin.
(i) 500 grs. of the coag-ulum completely dried at 212° left a residue of 143.6 grs.
500 : 528.6 : : 143.6 : x (= 151.81) solids in the clot.
(k) 528.6 - 151.8 = 376.8 water in the clot. Supposing the water entirely due to the serum with which the clot is penetrated, we should find it contain Since by (b) 906.55 : 376.8 : : 93.45 : a. (= 38.86) solids of the serum retained by the clot, and 376.8 + 38.86 = 415.66 weight of the serum retained.
But 415.66 -1- 471.4 by (a) = 887.06 total serum in 1000 parts of blood.
(/) 20 grs. of the dried matter of the coagu lum ignited left a red ash, weighing 0.52 grs. 20 : 151.81 : : 0.52 : x (= 3.947) salts in the clot.
But 1000:471.4 : : 7.3 by (c) : x (= 3.441) salts in the exuded serum.
3.947 -I- 3.441 = 7.388 salts in 1000 parts of blood.
(m) Since the coagulum contains 2.962 fibrin, And the serum retained 38.860 solids.
The sum of the two 41,822 And by deducting this sum from the total solids contained in the clot we find 151.81 - 41.822 = 109.988 red particles in 1000 (f blood.
(n) In order therefore to deduce the compo sition of 1000 parts of blood, we have only to calculate the following proportions from our knowledge of the composition of the serum.
1000 : 887.06 : 906.55 : (= 804.164) water.
1000 : 887.06 : : 81.974 : x (= 72.716) albumen.
1000 : 887.06 : : 1.900 : x (= 1.685) alco holic extract.
1000 : 887.06 : : 0.483 : x (= 0.428) watery extract.
1000: 887.06 : : 0.24 : (= 0.213) oily fat. To this we must add 0 04 from the fibiii making the total oilyfat = 0.253.
. 1000 : 887.06 : : 0.46 : x (= 0.408) crys talline fat .
1000 parts of this blood therefore consist of Water ....... .. . 804.164 Fixed salts 7.388 cRed particles 109.988 flbumen 72.716 Fibrin 2.962 Organic matters, Alcoholic extract 1.685 188.440. Watery extract 0.428 Oily fat .... 0.253 Crystalline fat 0.408 999.992 3. Analysts of milk.
Occasionally we may have to perform an analysis of mill( ; we may proceed as in the following instance.
The milk was raiher thin, watery in appear ance, and had a sp. gr. of 1031. It was ob tained from a woman aged 25, three weeks after the birth of her fourth child.
(a) 100 grs. evaporated to dryness left 11.49 grs. of solid matter.
100 : 1000 : 11.49 : x (= 114.9) solids per 1000.
1000 -114.9 = 885.1 water per 1000.
(b) On incinerating the residue, an alkaline ash was left amounting to 0.24 grs. = salts 2.4 per 1000.
(c) 158 grs. of the milk were mixed with a few drops of acetic acid and evaporated to dry ness, and digested repeatedly in ether (the ether was first allowed to macerate upon the residue unpowdered. It was decanted and the greater part of the fat thus removed; the residue was completely dried, powdered, and again subjected to three or four digestions with ether. All the ethereal solutions were then evaporated.) The fatty matter amounted to 4.61 grs.
158 : 1000 : : 4.61 : x (= 29.13) butter per 1000.
(d) The portion insoluble in ether was di gested in dilute alcohol (sp. gr. 920) as long as any thing dissolved. The solution on eva poration yielded a yellowish granular mass, consisting of milk sugar, and a little extractive matter : it amounted to 9.7 grs. and appeared perfectly free from casein.
158 : 1000 : : 9.7 : x (= 61.39) sugar of milk per 1000.
(e) The insoluble residue consisted almost entirely of casein, with a small quantity of sa line matter. Calculating by the deficiency, (as, owing to an accident, it was not weighed,) it amounted to 3.85 grs.